Hilser Laboratory Publications
Schrank, T., Bolen, D.W., and V.J. Hilser (2009) Rational Modulation of Conformational Fluctuations in Adenylate Kinase Reveal a Local Unfolding Mechanism for Allostery and Functional Adaptation in Proteins. Proc. Nat. Acad. Sci. USA. 106, 16984-16989.
Vertrees, J., Wrabl, J.O., and V.J. Hilser (2009) An Energetic Representation of Protein Architecture that is Independent of Primary and Secondary Structure. Biophys. J. 97, 1461-1470.
Gu, J. and V.J. Hilser (2009) Sequence-based Analysis of Protein Energy Landscapes Reveals Non-uniform Thermal Adaptation within the Proteome. Mol. Biol. Evol. 26, 2217-2227.
Manson, A., Whitten, S.T., Ferreon, J.C., Fox, R.O. and V.J. Hilser (2009) Characterizing the Role of Ensemble Modulation in Mutation-induced Changes in Binding Affinity J. Am. Chem. Soc. 131, 6785-6793.
Gu, J. and V.J. Hilser (2009) The Significance and Impacts of Protein Disorder and Conformational Variants. In Structural Bioinformatics, Second Edition. Ed. J. Gu and P.E. Bourne, John Wiley & Sons .
Vertrees, J., Wrabl, J.O., and V.J. Hilser (2009) Energetic Profiling of Proteins. Meth. in Enzym., 455, 299-327.
Hilser, V.J. and S.T. Whitten (2009) Energy Flow and Allostery in an Ensemble. In Proteins: Energy, Heat and Signal Flow Eds. David M. Leitner and John E. Straub. CRC Press, Taylor & Francis Group.
Gu, J and V.J. Hilser (2008) Predicting the Energetics of Conformational Fluctuations in Proteins from Sequence: A Strategy for Profiling the Proteome. Structure, 16, 1627-1637.
Wang, S., Gu, J., Larson, S.A., Whitten, S.T., and V.J. Hilser (2008) Denatured State Energy Landscapes of a Protein Structural Database Reveal the Energetic Determinants of a Framework Model for Folding. J. Mol. Biol. 381, 1184-1201.
Whitten, S.T Yang, H.-W. Fox, R.O., and V.J. Hilser (2008) Exploring the effect of conformational bias on the binding of peptides to the SEM5 C-SH3 domain. Prot. Sci. 17, 1200-1211.
Sayar K., Ugur, O., Liu, T., Hilser, V.J., Onaran, O. (2008) Exploring Allosteric Coupling in the alpha-Subunit of Heterotrimeric G Proteins Using Evolutionary and Ensemble-Based Approaches. BMC Structural Biology, 8:23.
Whitten, S.T., Garcia-Moreno E.,B., and V.J. Hilser (2008) Ligand Effects on the protein ensemble:Unifying the descriptions of ligand binding, local conformational fluctuations, and protein stability. Meth. Cell. Biol. 84, 871-891.
Whitten, S.T., Ferreon, J.C., Hamburger, J.B., and V.J. Hilser (2008) Calorimetric Determination of the Thermodynamics of Polyproline II (PII) Helix Formation in the Unfolded States of Protein. In Unfolded Proteins: From Denatured to Intrinsically Disordered Ed. Trevor Creamer, Nova Science Publishing.
Hilser, V. J. and E. B. Thompson (2007) Intrinsic Disorder as a Mechanism to Optimize Allosteric Coupling in Proteins. Proc. Nat. Acad. Sci. USA. 104, 8311-8315.
Liu, T., Whitten, S.T., and V.J. Hilser (2007) Functional Residues Serve a Dominant Role in Mediating Cooperativity of the Protein Ensemble Proc. Nat. Acad. Sci. USA. 104, 4347-4352.
Whitten, S. T.; Kurtz, A. J.; Pometun, M. S.; Wand, A. J.; Hilser, V. J. (2006) Revealing the Nature of the Native State Ensemble through Cold Denaturation. Biochemistry, 2006; 45(34); 10163-101
Hilser, V.J. , Garcia-Moreno E.,B., Oas, T.G, Kapp, G. and S.T. Whitten. (2006) A Statistical Thermodynamic Model of the Protein Ensemble. Chem. Rev., 106, 1545-1558.
Fitch, C.A., Whitten, S.T., Hilser, V.J. and Garcia-Moreno E.,B. (2006) Molecular Mechanism of pH-driven Conformational Transitions of Proteins: Insights from Continuum Electrostatics Calculations of Acid Unfolding. Proteins, 63, 113-126.
Liu, T., Whitten, S.T., and V.J. Hilser (2006) Ensemble-based Signatures of Energy Propagation in Proteins. A New View of an Old Phenomenon. Proteins, 62, 728-738.
Whitten, S.T., Garcia-Moreno E.,B., and V.J. Hilser (2005) Local Fluctuations Can Modulate the Coupling Between Proton Binding and Global Structural Transitions in Proteins. Proc. Nat. Acad. Sci. USA. 102, 4282-4287.
Vertrees, J., Barritt, P., Whitten, S. and Hilser, V.J. (.2005) COREX/BEST server: a web browser-based program that calculates regional stability variations within protein structures. Bioinformatics. Aug 1;21(15):3318-9.
Ferreon, J.C., Hamburger, J. B. and V.J. Hilser (2004) An Experimental Strategy to Evaluate the Thermodynamic Stability of Highly Dynamic Binding Sites in Proteins. J. Am. Chem. Soc. 126, 12774-12775.
Babu, C.R., Hilser, V.J. and A. J. Wand (2004) Direct Access to the Cooperative Substructures of Proteins and the Protein Ensemble via Cold Denaturation. Nat. Struct. Mol. Bio. 11, 352-357.
Hamburger, J. B. Ferreon, J.C., Whitten, S.T and V.J. Hilser (2004) Thermodynamic Origins and Consequences of the Polyproline II (PII) Conformational Bias in the Denatured States of Proteins. Biochemistry. 43,9790-9799.
Ferreon, J.C. and V.J. Hilser. (2004) Thermodynamics of Binding to SH3 Domains: The Energetic Impact of Polyproline II (PII) Helix Formation. Biochemistry. 43, 7787-7797.
Larson, S.A., and V.J. Hilser. (2004) Analysis of the ‘Thermodynamic Information Content’ of a Homo sapiens Structural Database Reveals Hierarchical Thermodynamic Organization. Prot. Sci. 13, 1787-1801.
Ferguson, H.R., Fan,, X. Mukherjee, M., Luo, J., Khan, R., Ferreon, J.C., Hilser, V.J., Shope, R.E. and R.O. Fox. (2004) Directed Discovery of Bivalent Peptide Ligands to an SH3 Domain. Prot. Sci. 13, 626.
Ferreon, J.C., Volk, D.E., Luxon, B.A., Gorenstein, D.G., and V.J. Hilser.(2003) Solution Structure, Dynamics and Thermodynamics of the Native State Ensemble of the SEM5 C-terminal SH3 Domain. Biochemistry.42, 5582-5591.
Ferreon, J.C. and V.J. Hilser (2003) Ligand-induced changes in dynamics in the RT loop of the C-terminal SH3 domain of SEM-5 indicate cooperative conformational coupling. Prot. Sci. 12. 982-996.
Ferreon, J.C. and V.J. Hilser (2003) The Effect of the Polyproline II (PPII) Conformation on the Denatured State Entropy. Prot. Sci. 11, 447-457.
Wrabl, J.O., Larson, S.A., and V.J. Hilser. (2002) Thermodynamic Environments in Proteins: Fundamental Determinants of Fold Specificity. Prot. Sci. 11,1945-1957.
Wrabl, J.O., Larson, S.A., and V.J. Hilser. (2001) Thermodynamic Propensity of Amino Acids in the Native State Ensemble: Implications for Fold Recognition. Protein Sci. 10, 1032-1045 .
Whitten, S.T., Wooll, J.O., Razeghifard, R., Garcia-Moreno E.B., and V.J. Hilser. (2001) The origin of pH-dependent changes in m -values for the denaturant-induced unfolding of proteins. J. Mol. Biol 309, 1165-1175.
Hilser, V.J.. (2001) Modeling the Native State Ensemble. In Protein Structure, Stability and Folding. Methods in Molecular Biology. Ed. K. Murphy. Humana Press, Totowa, NJ, 93-115.
Pan, H., Lee, J.C. and V.J. Hilser. (2000) Binding Sites in Escherichia Coli Dihydrofolate Reductase Communicate by Modulating the Conformational Ensemble. Proc. Nat. Acad. Sci. USA 97, 12020-12025.
Wooll, J.O., Wrabl, J.O. and V.J. Hilser. (2000)Ensemble Modulation as an Origin of Denaturant Independent Hydrogen Exchange in Proteins. J. Mol. Biol. 301, 247-256.
Previous Hilser Publications (Graduate and Post-Doctoral)