Michal R. Szymanski

Lab:

Dr. Wlodek Bujalowski

In BMB since (year):

MBET 2007

Biographical Information:

• Undergraduate institution:
  University of Houston
• Undergraduate degree:
  Bachelor of Science in Biochemistry and Biophysics

Research interests:

I am interested in protein-protein and protein-nucleic acid interactions. Currently, I am working on the assembly process of the primosome.

Interests outside of the lab:

Volleyball, soccer, karate, basketball, horseback riding, rock-climbing. I enjoy listening to the music, playing guitar, cooking.

Grants/Awards:

• Robert A. Welch Award for Excellence in Graduate Research in Chemistry (2009)
• Barbara Bowman Memorial Award for Research Excellence (2009)
• Robert A. Welch Foundation Pre-Doctoral Fellowship Recipient (2006 and 2007)
• Sigma Xi, The Scientific Research Society Poster Competition. The Best Student Poster Award (2006)

Publications/Posters:

1. Szymanski, M.R., Jezewska, M.J., Bujalowski, W. The Escherichia coli PriA Helicase-Double-Stranded DNA Complex: Location of the Strong DNA-Binding Subsite on the Helicase Domain of the Protein and the Affinity Control by the Two Nucleotide-Binding Sites of the Enzyme. J. Mol. Biol. 2010 Jun 16. [Epub ahead of print]
2. Szymanski, M.R., Jezewska, M.J., Bujalowski, W. Interactions of the Escherichia coli Primosomal PriB Protein with the Single-Stranded DNA. Stoichiometries, Intrinsic Affinities, Cooperativities, and Base Specificities. J. Mol. Biol. 398(1):8-25. 2010.
3. Szymanski, M.R., Jezewska, M.J., Bujalowski, W. The E. coli PriA helicase specifically recognizes gapped DNA substrates. Effect of the two nucleotide-binding sites of the enzyme on the recognition process. J. Biol. Chem. 285(13):9683-96. 2010.
4. Andreeva, I.E., Roychowdhury, A., Szymanski, M.R., Jezewska, M.J., Bujalowski, W. Mechanisms of Nucleotide Cofactor Interactions With the RepA Protein of Plasmid RSF1010. Binding Dynamics Studied Using Fluorescence Stopped-Flow Method. Biochemistry. 48(44):10620-36; 2009.
5. Szymanski, M.R., Fiebach, A.R., Tratschin, J-D., Gut, M., Ramanujam, V.M., Gottipati, K., Patel, P., Ye, M., Ruggli, N., Choi, K.H. Zinc-Binding in Pestivirus Npro is Required for Interferon Regulatory Factor 3 (IRF3) Interaction and Degradation. J. Mol. Biol. 391(2):438-49; 2009.
6. Roychowdhury, A., Szymanski, M.R., Jezewska, M.J., Bujalowski, W. Interactions of the E. coli DnaB - DnaC Protein Complex with Nucleotide Cofactors. I. Allosteric Conformational Transitions of the Complex. Biochemistry. 48(29):6712-29; 2009.
7. Roychowdhury, A., Szymanski, M.R., Jezewska, M.J., Bujalowski, W. Mechanism of NTP Hydrolysis by the Escherichia coli Primary Replicative Helicase DnaB Protein. II. Nucleotide and Nucleic Acid Specificities. Biochemistry. 48(29):6730-46; 2009.
8. Roychowdhury, A., Szymanski, M.R., Jezewska, M.J., Bujalowski, W. The Escherichia coli DnaB Helicase - DnaC Protein Complex: Allosteric Effects of the Nucleotides on the Nucleic Acid Binding and the Kinetic Mechanism of NTP Hydrolysis. III. Biochemistry. 48(29):6747-63; 2009.
9. Andreeva, I.E., Szymanski, M.R., Jezewska, M.J., Galletto, R., Bujalowski, W. Dynamics of the ssDNA Recognition by the RepA Hexameric Helicase of Plasmid RSF1010: Analyses Using Fluorescence Stopped-Flow Intensity and Anisotropy Methods. J. Mol. Biol. 388(4):751-75; 2009.
10. Duret, G., Szymanski, M., Choi, K.J., Yeo, H.J., Delcour, A.H. The TpsB translocator HMW1B of haemophilus influenzae forms a large conductance channel. J. Biol. Chem. 283(23):15771-8; 2008.