Pomila Singh, Ph.D., Professor
Dr. Singh's laboratory is conducting research in the area of hormones and growth factors as it relates to cancer. Dr. Singh is a full Professor in the Departments of Neuroscience & Cell Biology, Biochemistry & Molecular Biology and Medicine. Her laboratory has published more than 105 papers, and she has trained several postdoctoral fellows and graduate students.Dr. Singh's laboratory is currently funded by several NIH grants to investigate the role of autocrine growth factors (gastrin, IGF-II, IGF-BPs) in the growth and differentiation of cancer cells.On-going projects involve the generation of knockout and transgenic mice, investigation of signal transduction and apoptotic pathways, generation of tumor vaccines, gene therapy, receptor characterization, study of role of growth factors in carcinogenesis, identification of differential gene expression at different stages, and transcriptional regulation of specific genes.
FigureLegend: Transcriptional factors and cis elements known to bind the proximal and distal segments of IGF-II P3 promoter.
The ~ nt position of the cis elements along the promoter is indicated.+ = up-regulation of promoter activity; - = down-regulation of promoter activity.A = cis element in the proximal promoter region of the IGF-II P-3 promoter.B = cis element recently identified by our laboratory in the distal segment of the P3 promoter. The approximate position of the transcriptional factors that have been shown to bind to the indicated cis elements in the proximal and distal segments of the P3 promoter are indicated.Wt/egr = wilms tumor antigen 1/early growth response element; IPBP4/5 = transcriptional factors that bind the novel cis element IPBP4/5 as described in References 58,59; AP-2 = activating protein2; Sp1 = Sp1 transcriptional factors, binding GC rich areas; CTF = transcriptional factors binding a novel cis element as described in Reference 59; TBP = TATA box binding proteins; P53 = tumor suppressor protein; TFs = transcriptional factors; P3-D = a novel cis element described by us in J. Biol. Chem. 276: 6937-6944, 2001; CORE = represents the core elements required for optimal activation of the P3 promoter; REG = regulatory elements; EXON 5 = the EXON 5 of IGF-II human promoter.